Q5 dna polymerase protocol
How to Choose the Right DNA Polymerase for PCR

Choosing the correct dna polymerase for pcr depends on many factors. Neb offers a range of dna polymerase formulations optimized for different...



Tutorial 4: QuikChange site-directed mutagenesis (Stratagene)


Plasmid mutagenesis made easy. Make sure to use a Tm calculator to check the melting temperatures of your primers:...



Site-directed Mutagenesis

Site-directed mutagenesis involves making localized edits to a preexisting dna sequence. Typically it is used to introduce point mutations into a sequence such...



Dr. Dailey REMO Protocol Update - ePCR Transfer of Care Snippet

New remo Protocol update and nysdoh requirements for transfer of care and timely (within 2 hours of tot care to ED) submission of pcr document.



Site-Directed Mutagenesis (Day One)

High Tech High - Student Biology Video Guides.



Principle of site-directed mutagensis by PCR


Correction: parental plasmids are methylated and are digested by DpnI. For Stragene Quickchange protocol, two complimentary primers containing the...



Real time PCR using molecular beacon

For more information, log on to Download the study materials here- A...



OligoMix General Use Considerations

OligoMix is a versatile, innovative, custom product for genomics discoveries. We synthesize thousands of oligonucleotide sequences at once in massive...



Reverse


understandind biochemistry. Reverse transcription from mrna to double strand dna catalized by reverse transkciptase.



Introduction to Gibson Assembly


Watch an interactive tutorial that details the process by which Gibson Assembly joins dna fragments in a single tube, isothermal reaction.